Specifications @Reproduction and Genetics
a. Lasers: 405, 488, 560, 640
b. 3 detectors: 2 GaAsP PMTs, one Airyscan detector for superresolution and increased sensitivity (4-8x).
c. Objectives : EC Plan Neofluar 10x/0.3 NA; EC Plan Neofluar 20x/0.5 NA; Plan Apochromat 20x/0.8 NA; LD C-Apochromat 40x/ 1.1 NA water immersion; Plan Apochromat 63x/1.4 oil
d. Axiocam 503 mono camera for widefield mode
e. Tokai Hit incubator. Multiwell plates, Labtek, 35 and 50 mm dishes can be used, plus your special 4 well dishes.
f. Motorized stage: multi position and tiles imaging possible.
g. LED 780 nm excitation combined with proper filter set for imaging NIR fluorophores such as ICG.
h. Software options: Multi channel fluorescence, z-stack, time lapse microscopy , superresolution microscopy via Airyscan, time lapse microscopy, Tiles and positions, Experiment designer for complex experiments, spectral imaging with linear unmixing, image analysis.
i. Price without Airyscan: 25 Euro/hour
j. Price with Airyscan: 30 Euro/hour
Specifications @Follicle Biology
a. Lasers: 405, 488, 560, 640
b. 2 multi alkali PMTs
c. Tokai Hit incubator
Specifications
a. Lasers: 405, 488, 560, 640
b. 1 detector- PCO Edge very sensitive and fast CMOS camera
c. Objectives : Illumination- 2 5x objectives and 2 10x objectives. Detection objectives: EC Plan Neofluar 5x/ 0.16 , Water Plan Apochromat 10x/0.5 NA; Water Plan -Apochromat 20x/ 1.0 NA for living samples; Water Plan Neofluar 20x/ 1.0 NA for cleared 1.45 refractive index samples
d. Incubation: temperature control, CO2, and O2 control
e. Software options: Multi channel fluorescence, z-stack, time lapse microscopy, Multiview imaging, and tiles imaging. Offline workstation with powerful Arivis for data processing, 36Tb capacity.
f. Price consortium member: 35 Euro/hour
g. Price Non-consortium member: 45 Euro/hour
Specifications
a. Adjustable-intensaty LED light cubes: RFP, Texas Red, GFP and DAPI
b. High-sensitive 3.2 MPCMOS sensor with 3.45µm pixel resolution.
c. Objectives: FL 20x LWDPH 0.45NA/6.12WD FL 10x LWDPH 0.30NA/7.13WD FL 4x LWDPH 0.13NA/10.58WD
d. Incubator: humidity, temperature, CO2 and O2 control
e. Motorized stage : different inserts for plates and slides.
f. Software options: Full well imaging, full plate imaging, Celleste software for deconvolution and image processing.
g. Price without incubator: 12Euo/hour
h. Price with incubator: 15 Euro/hour
General rules
- A maximum of 4 hours per day can be booked during the working hours (9-5), exception can be made for life imaging or outside of working hours.
- If there would be more timeslots it is possible to extend past the 4 hours in consultation with our technician (Brecht Ghesquiere).
- Users can only book 2 weeks in advance.
- Upon training with our technician, you can request an invite to view the calendar.
- You can book time on one of the microscopes by email to our technician (Brecht Ghesquiere).
- When you arrive or leave for use of the microscope, please inform the technician (Brecht Ghesquiere) of your arrival/departure.
- Upon arrival you must sign the logbook and again when you leave.
- Minimum booking time is half an hour.
- Reduction or prolongation of the booking can be arranged with our technician (Brecht Ghesquiere).
- If you fail to show up you will still be charged for hours booked.
- Workstation is available free of charge but must be booked through our technician (Brecht Ghesquiere), if help is needed for image analysis, our technician is available but you will be charged a small service fee of 15 €/h.
- The data will be stored up to 2 weeks after last imaging session.
- You can retrieve your data through the workstation or our technician (Brecht Ghesquiere) can send it to you.
- If you do not collect your data within those 2 weeks, we will DELETE your data.
Contact
1090 Jette, Laarbeeklaan 103, Building K, floor+1, follow signs for REGE.
Booking and information on charges via our trained technician Brecht Ghesquiere (brecht.ghesquiere@vub.be).
